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Risk analysis of pest infestation of wooden structures
Brada, Stanislav ; Hunková,, Veronika (referee) ; Šebesta, Petr (advisor)
The thesis deals with risks of defect occurrences in a wood construction. Wood constructions and their parts are exposed to a number of negative factors influencing their lifetime. The aim of the thesis is to give an outline of risks of defect occurrences in a wood construction attacked by pests and propose measures for their elimination.. These steps are closely described in the individual parts and processes. Part of the thesis is the FMEA analysis, resulting in appropriate chemical protection, a factor considered before incorporation into the construction. The analysis and its outcomes are important for improvement of the wood construction lifetime.
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Vliv aplikace insekticidů na slunéčko východní (Harmonia axyridis)
Kintlerová, Jarmila
The diploma thesis was focused on evaluating the influence of selected active ingredients of insecticides used to protect agricultural crops on populations of the invasive species of harlequin ladybird (Harmonia axyridis Pallas, 1773) obtained from various locations within the Czech Republic. Four active ingredients, acetamiprid, indoxacarb, lambda-cyhalothrin and tau-fluvalinate, were tested according to the IRAC methodology on populations of adult individuals collected at seven locations. The experiment was done in the form of adult vial test, with subsequent evaluation using the Polo Plus 2.0 software. Based on the obtained results of mortality and lethal doses of active ingredients, it was found that the populations were highly sensitive to acetamiprid and lambda-cyhalothrin and sensitive with possible emergence of resistance to tau-fluvalinate. In the case of indoxacarb, populations from three locations showed moderate to high resistance.
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Pěstování a odrůdový sortiment papriky roční
Suvorova, Mariya
Suvorova, M., Cultivation and variety of annual peppers. Bachelor thesis. Brno: Mendel University in Brno, 2021. The bachelor's thesis deals with the cultivation of a selected assortment of annual pepper (Capsicum annuum L.). The aim of the thesis is to describe the growing technology and agricultural technology, including protection against diseases and pests and to determine the growth and yield indicators for a selected range of annual peppers. The first part of the thesis is focused on the elaboration of a literature review related to the origin of the species, botanical characteristics, habitat requirements, cultivation technology and assortment of varieties. The second part contains a description of an experimental experiment in the cultivation of peppers with subsequent measurement and evaluation of yield indicators. The results obtained during the experiment were tabulated and explained. The final part of the thesis is devoted to the discussion of the results and comparison with the results from previous years.
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Vlnatka krvavá (Eriosoma lanigerum), význam a možnosti její regulace
Klimešová, Lada
The research was performed in vegetation season of year 2014 in apple orchard near Nosislav in Břeclav district. The task was to compare efficiency of two auxiliary preparations and two insecticides, separately or in different combinations. Valuation of efficiency of these preparations in various variants was performed by measuring lengths of colonies of woolly aphid 1.2 and 7 days after application. The trial had been started at 4.6.2014. The most efficient variant was Cocana 1.5% followed by Mospilan 20 SP 0.013% -- Mospilan was applied immediately after applying of Cocana (efficiency 80.2%). Very good results were noticed on variants Mospilan 20 0.013% (efficiency 75.6%), Cocana 2% + Mospilan 20 SP 0,013 % -- tankmix (74.2%), Cocana 2% followed by Mospilan 20 SP 0,013% (68.8%), Cocana 1% followed by Mospilan 20 SP 1% (64.5%). Low efficiency was found out at Cocana 2% followed by Wetcit 0.3% (48.7%). The worst efficiency was by application of Cocana 2% followed by Spintor 0.6 l/ha (13%).
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Prostorové rozmístění škůdců v porostu máku setého v okolí Rozseče
Špička, František
The occurrence of the opium poppy pests was observed in the Bohemian-Moravian highland around the village Rozseč in the years 2012 on two study areas, in the year 2014 on four study areas. There was discovered occurrence of three pests. The occur-rence of poppy root weevil exceeded threshold on all study areas. In the year 2012 one plant had occurrence on an average of 4.32--5.82 larvae. 3.15--5.18 adults on meter of line were registered, maximal amount was nine adults in the year 2014. The infestation intensity of plants was 29--61 %. 1.3--2.56 larvae on one plant were discovered, maxi-mum seven larvae. The root weevil occurred evenly on all area of all study areas. Weevil poppy was discovered on study area with lowest height above sea level (604 m) with west inclination only in the year 2014. Adults were registered on five poppy head. The occurrence of weevil poppy obviously was not disintegrating on all area. The black bean aphid occurred in the middle intensity in the year 2012. 4--11% infestation of plants was discovered after leaving overflight from the winter hosts in the year 2014. 17--41% infestation of plants was discovered before blossom. The irregular dispersion of the black bean aphid on a few lands could have been caused by wind.
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Výskyt obalečů na révě vinné a jejich regulace v podmínkách integrované produkce
Šnajder, Radek
Occurrence of tortricid moths was investigated during the growing season of 2013 in the district of Znojmo using pheromone traps in two vineyards in integrated production and in organic apple orchard. In both vineyards there was registered the occurrence of all three monitored species -- Eupoecilia ambiguella (Hubner, 1796), Lobesia botrana (Denis & Schiffermuller, 1775) and Sparganothis pilleriana (Denis & Schiffermuller, 1775). All species reached higher numbers in the study St. Urban. Lobesia botrana reached the highest numbers (152 total individuals). The first imago was captured on the 3rd of May, the last on the 17th of August. The maximum (26 individuals) was on the 15th of July. Lobesia botrana (76 total individuals) was for the first time identified on the 3rd of May, the last individual was identified on the 17th of August. The maxi-mum (17 individuals) was on the 15th of July. Sparganothis pilleriana (3 imagos) was registered only in vineyards on the 1st of July and on the 10th of July. Diachrysia chry-sitis (L., 1758), Oligia latruncula (Denis & Schiffermuller, 1775), Pasiphila rectangu-lata (L., 1758), Idaea biselata (Hunfagel, 1767) and Neosphaleroptera nubilana (Hüb-ner, 1799) were caught in the trap for Eupoecilia ambiguella. Hofmannophila pseudos-pretella (Stainton, 1849), Agrotis bigramma (Esper, 1790), Idaea biselata (Hunfagel, 1767) were caught in the trap for Lobesia botrana.
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Transformation of potato with protease inhibitor gene SPI-2
Říhová, Barbora ; Fischer, Lukáš (advisor) ; Vítámvás, Pavel (referee)
The subject of my thesis was to genetically modify a potato for increased resistance against its pathogens and pests. In developing a resistant plant, it is quite common to use the same type of molecules that plants use themselves in their defense reactions. In this work I used the gene SPI-2 originating from a honeycomb moth (Galleria mellonella). The protein SPI-2 is a member of serine protease inhibitors. Since the previous attempts of the team to detect the protein in transformed plants haven't been successful, the basic form of the gene was modified by adding a Kozak sequence near the start codon, which should have increased the translation initiation and hence increase the level of the protein. Two constructs were prepared for the transformation: SPI-2-T a SPI-2-Y. They differ by one amino acid, which slightly changes their inhibitory activity. First, the construct SPI-2-T was used for a transient transformation of tobacco Nicotiana benthamiana by agroinfiltration of its leaves. Then both constructs were used for a stable transformation of Solanum tuberosum cv. Desireé. The detection of the protein has not been successful, although the inserted gene was transcribed and his sequence was verified by sequencing. It is therefore most likely that the protein has a low stability in the cytoplasm....
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